ScholarLens Back to search
2009•China Animal Husbandry & Veterinary MedicineRequires access

PCR-SSCP Analysis on AHCY Gene in Holstein Cows

Fang Li

Open publisher page 0 citations

Abstract

The AHCY(S-adenosylhomocysteine hydrolase) gene was studied as a candidate gene for mastitis resistance in 210 Holstein cows.Single nucleotide polymorphisms(SNPs) of all exons(exons 1 to 10) of AHCY gene were detected with thirteen pairs of primers by polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) in 210 Holstein cows.No polymorphism was detected in the amplified region in the ten exons tested,this result indicated that the genetic markers that affect the mastitis resistance in Holstein cows may not exist in the detected exons of AHCY gene.

About this research paper

What this paper is about

The AHCY(S-adenosylhomocysteine hydrolase) gene was studied as a candidate gene for mastitis resistance in 210 Holstein cows.Single nucleotide polymorphisms(SNPs) of all exons(exons 1 to 10) of AHCY gene were detected with thirteen pairs of primers by polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) in 210 Holstein cows.No polymorphism was detected in the amplified region in the ten exons tested,this result indicated that the genetic markers that affect the mastitis resistance in Holstein cows may not exist in the detected exons of AHCY gene.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

The AHCY(S-adenosylhomocysteine hydrolase) gene was studied as a candidate gene for mastitis resistance in 210 Holstein cows.Single nucleotide polymorphisms(SNPs) of all exons(exons 1 to 10) of AHCY gene were detected with thirteen pairs of primers by polymerase chain reaction-single strand conformation polymorphism(PCR-SSCP) in 210 Holstein cows.No polymorphism was detected in the amplified region in the ten exons tested,this result indicated that the genetic markers that affect the mastitis resistance in Holstein cows may not exist in the detected exons of AHCY gene.

Key concepts: Exon, Single-nucleotide polymorphism, Biology, Single-strand conformation polymorphism, Genetics, Gene, Polymerase chain reaction, Mastitis

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
PCR-SSCP Analysis on AHCY Gene in Holstein Cows — Research Paper | ScholarLens