SSCP Polymorphism Research of Promoter of FcRn α Chain Gene in Holstein Dairy Cows

The PCR-SSCP polymorphisms of promoter of Fc receptor (FcRn) α chain (FCGRT) gene were studied in 189 Holstein cows. The results showed that PCR-SSCP polymorphisms were identified in promoter amplified fragment (P1,P2 and P3) of FCGRT gene. For the amplified fragment of P1,the genotype frequency of AA,AB and BB was 25.40%,59.26% and 15.34%,respectively. For the amplified fragment of P2,the genotype frequency of CC,CD and DD was 20.63%,56.61% and 22.75%,respectively. For the amplified fragment of P3,the genotype frequency of EE,EF and FF was 1.59%,12.17% and 86.24%,respectively.The sequencing analysis indicated that the polymorphisms were separately due to three single point mutation C to T,C to A and T to A at amplified fragment. Except for SNP1 in Holstein cows,the SNP2 and SNP3 sites in the populations were in a state of Hardy-Weinberg equlibrium.