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2009•China Medical HeraldRequires access

Determination of Vitamin C in Jueming Jiangzhi Tablets by HPLC

Yu Yan

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Abstract

Objective: To establish a method for the determination of Vitamin C in Jueming Jiangzhi Tablets. Methods: A Hypersil BDS C18 column(4.6 mm×200 mm,5 μm) was used with a mobile phase of 1% phosphoric acid, the flow rate was 0.8 ml/min, the detection wavelength was set at 244 nm. Results: The standard curves was linear in the rang of 0.089-0.801 μg(r=1.000 0), the average recovery was 99.99% with a RSD of 0.08%. Conclusion: The method is simple, accurate and reproducible, and is suitable for quality control of this tablets.

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What this paper is about

Objective: To establish a method for the determination of Vitamin C in Jueming Jiangzhi Tablets. Methods: A Hypersil BDS C18 column(4.6 mm×200 mm,5 μm) was used with a mobile phase of 1% phosphoric acid, the flow rate was 0.8 ml/min, the detection wavelength was set at 244 nm. Results: The standard curves was linear in the rang of 0.089-0.801 μg(r=1.000 0), the average recovery was 99.99% with a RSD of 0.08%. Conclusion: The method is simple, accurate and reproducible, and is suitable for quality control of this tablets.

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Available abstract

Objective: To establish a method for the determination of Vitamin C in Jueming Jiangzhi Tablets. Methods: A Hypersil BDS C18 column(4.6 mm×200 mm,5 μm) was used with a mobile phase of 1% phosphoric acid, the flow rate was 0.8 ml/min, the detection wavelength was set at 244 nm. Results: The standard curves was linear in the rang of 0.089-0.801 μg(r=1.000 0), the average recovery was 99.99% with a RSD of 0.08%. Conclusion: The method is simple, accurate and reproducible, and is suitable for quality control of this tablets.

Key concepts: Medicine, Phosphoric acid, Chromatography, High-performance liquid chromatography, Content determination, Linear relationship, Organic chemistry, Chemistry

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