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Determination of tetrahydropalmatine content in Zhuanggu Zhitong granule by HPLC method

Wang Ju-cun

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Abstract

Objective: To determine tetrahydropalmatine content in Zhuanggu Zhitong granule by HPLC.Methods:Symmetry C18 column(150 mm×3.9 mm,5 μm) was used.Acetonitrile/ 0.1% phosphoric acid(adjusted pH to 6.0 with triethylamine)(34/66) was used as the mobile phase,the flow rate was 0.8 mL.min-1,the detected wavelength was 280 nm,and the temperature of column was controlled at room temperature.Results:The calibration curve was linear in the range of 0.005～0.025 mg.mL-1for tetrahydropalmatine(r=0.999 6).The average recovery was 99.5%(n=6),RSD was 1.35%.Conclusion:The method is simple,accurate and reliable,and can be used to determine the concentration of tetrahydropalmatine content in Zhuanggu Zhitong granule.

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What this paper is about

Objective: To determine tetrahydropalmatine content in Zhuanggu Zhitong granule by HPLC.Methods:Symmetry C18 column(150 mm×3.9 mm,5 μm) was used.Acetonitrile/ 0.1% phosphoric acid(adjusted pH to 6.0 with triethylamine)(34/66) was used as the mobile phase,the flow rate was 0.8 mL.min-1,the detected wavelength was 280 nm,and the temperature of column was controlled at room temperature.Results:The calibration curve was linear in the range of 0.005～0.025 mg.mL-1for tetrahydropalmatine(r=0.999 6).The average recovery was 99.5%(n=6),RSD was 1.35%.Conclusion:The method is simple,accurate and reliable,and can be used to determine the concentration of tetrahydropalmatine content in Zhuanggu Zhitong granule.

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Available abstract

Objective: To determine tetrahydropalmatine content in Zhuanggu Zhitong granule by HPLC.Methods:Symmetry C18 column(150 mm×3.9 mm,5 μm) was used.Acetonitrile/ 0.1% phosphoric acid(adjusted pH to 6.0 with triethylamine)(34/66) was used as the mobile phase,the flow rate was 0.8 mL.min-1,the detected wavelength was 280 nm,and the temperature of column was controlled at room temperature.Results:The calibration curve was linear in the range of 0.005～0.025 mg.mL-1for tetrahydropalmatine(r=0.999 6).The average recovery was 99.5%(n=6),RSD was 1.35%.Conclusion:The method is simple,accurate and reliable,and can be used to determine the concentration of tetrahydropalmatine content in Zhuanggu Zhitong granule.

Key concepts: Tetrahydropalmatine, Granule (geology), Triethylamine, Chromatography, Calibration curve, Chemistry, Content determination, High-performance liquid chromatography

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