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Expression of Streptococcus suis serotype 2 gene mrp in attenuated Salmonella X4072

Zhao-Shan Zhang

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Abstract

The muramidase-released protein gene of Streptococcus suis serotypes 2 was isolated by PCR with the Streptococcus suis serotypes 2 strain from Sichuan in China,and ligated to the pMD18-T vector.The antibiotic resistance gene ampr of pMD18-T vector was destroyed by insertion of asd gene.Then the recombinant vector was transformed in Salmonella X4072 (asd-).The results of PCR,restriction enzyme digestion and SDS-PAGE indicated MRP was expressed by Salmonella X4072.

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What this paper is about

The muramidase-released protein gene of Streptococcus suis serotypes 2 was isolated by PCR with the Streptococcus suis serotypes 2 strain from Sichuan in China,and ligated to the pMD18-T vector.The antibiotic resistance gene ampr of pMD18-T vector was destroyed by insertion of asd gene.Then the recombinant vector was transformed in Salmonella X4072 (asd-).The results of PCR,restriction enzyme digestion and SDS-PAGE indicated MRP was expressed by Salmonella X4072.

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Available abstract

The muramidase-released protein gene of Streptococcus suis serotypes 2 was isolated by PCR with the Streptococcus suis serotypes 2 strain from Sichuan in China,and ligated to the pMD18-T vector.The antibiotic resistance gene ampr of pMD18-T vector was destroyed by insertion of asd gene.Then the recombinant vector was transformed in Salmonella X4072 (asd-).The results of PCR,restriction enzyme digestion and SDS-PAGE indicated MRP was expressed by Salmonella X4072.

Key concepts: Streptococcus suis, Biology, Serotype, Salmonella, Microbiology, Gene, Recombinant DNA, Virology

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