Isolation and Identification on Pathogens of Swine Streptococcosis and PCR Assays for Detection of Virulence Factors of Streptococcus suis Serotype 2

Gram-postive bacterium,identificated with Streptococcus suis serotype 2 by Rapid ID 32 Strep reagent of Streptococcus suis,was isolated in Guangxi.PCR assays were performed for detection of cps2J and three virulence factors,muramidase-relseased protein(MRP),extracelluar factor(EF) and suilysin gene(SLY) of Streptococcus suis.The sequence analysis revealed 98.8% homology to that of cps2J in GenBank.Virulence factors of Streptococcus suis serotype 2 were sly+mrp+ef+,which indicated that the cause of acute death of pigs was high-virulence Streptococcus suis serotype 2 in the pig farm.It was showed that determined bacteria caused infected rats death and leaded to infected rabbits showing temperature hoist to 40.3 ℃ and death due to blood poisoning.