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2002•Lishizhen Medicine and Materia Medica ResearchRequires access

Determination of Ferulic Acid in Duodongan Oral Solution by HPLC

Tan Xuan-zhong

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Abstract

Objective:To use a PR-HPLC method for determination of ferulic acid in Liquid of Duodongan.Methods:The sample was prepared by macroporous resin DA 201. The determination was carried out on Spherisob C 18 ODS (5 μm,4.6 mm×300 mm) column with mobile phase of MeOH-1% acetic acid (4∶6).Results:Sample size showed a good linear relationship at the range of 0.01～0.09 μg and correlation coefficient was 0.999 7. The average recovery of added sample was 96.69% (n=5). RSD was 1.0%.Conclusion:The method is simple, accurate, reproducible.

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What this paper is about

Objective:To use a PR-HPLC method for determination of ferulic acid in Liquid of Duodongan.Methods:The sample was prepared by macroporous resin DA 201. The determination was carried out on Spherisob C 18 ODS (5 μm,4.6 mm×300 mm) column with mobile phase of MeOH-1% acetic acid (4∶6).Results:Sample size showed a good linear relationship at the range of 0.01～0.09 μg and correlation coefficient was 0.999 7. The average recovery of added sample was 96.69% (n=5). RSD was 1.0%.Conclusion:The method is simple, accurate, reproducible.

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Available abstract

Objective:To use a PR-HPLC method for determination of ferulic acid in Liquid of Duodongan.Methods:The sample was prepared by macroporous resin DA 201. The determination was carried out on Spherisob C 18 ODS (5 μm,4.6 mm×300 mm) column with mobile phase of MeOH-1% acetic acid (4∶6).Results:Sample size showed a good linear relationship at the range of 0.01～0.09 μg and correlation coefficient was 0.999 7. The average recovery of added sample was 96.69% (n=5). RSD was 1.0%.Conclusion:The method is simple, accurate, reproducible.

Key concepts: Ferulic acid, Chromatography, High-performance liquid chromatography, Chemistry, Acetic acid, Correlation coefficient, Linear correlation, Linear relationship

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