Screening of Marine Producing Chitinase Microbes and Optimization of Fermentative Combination

[Objective]The aim was to explore the optimum fermentative combination of marine producing chitinase microbes.[Method]2 strains with strong chitinase activity were isolated from the sea mud from Caofeidian port area of Tangshan harbor and the effect of the different culture condition,induction and combined fermentation on producing chitinase of strain were studied.[Result] The transparent circle of the strain produced by UV mutation was bigger and the diameter was 3-4 mm,but the quantity was less.The colloidal chitin was the best carbon source of medium and the optimum temperature for producing chitinase was 32 ℃;The yield of enzyme was highest when the pH value was between 7.0 and 8.0;the effect of using peptone and yeast as nitrogen source on producing chitinase was obvious;the producing enzyme capability in the culture medium adding Mn2+ was strongest;after the induced strain produced enzyme through combined fermentation,the chitinase activity was increased significantly with the increment of the culture time.[Conclusion] In combined fermentation,Selecting the culture condition of the enzyme with high producing ability and mixed culture with different source strain had great meaning for combined fermentation.