Determination and entrapment efficiency of docetaxel liposomes for injection by ultrafiltration-HPLC

Objective To study on the determination and entrapment efficiency of docetaxel liposomes for injection.Methods Docetaxel liposomes were prepared with emulsify-volatilizing method,and the free drug were separated from liposomes with ultrafiltration.The content and entrapment efficiency of docetaxel liposomes were determined by HPLC.HPLC conditions were as follows: Agilent Tc-C18(4.6 mm×250 mm,5 μm);eluting with methanol-acetonitrile-water(35:40:25)at a flow rate of 1.0 mL·min-1,detecting at 233 nm wavelength,20 μL injection volume.Results The free docetaxel was separated from the liposomes with ultrafiltration,in which the blank recovery rate was 96.5%,and the sample recovery rate was 98.3%.Accessories don't affect the determination of docetaxel in these chromatographic conditions;It has a good linearity in a range of 3～300 μg·mL-1of docetaxel,and the recovery ratio was 97.06%～102.19%.The content of docetaxel liposomes was 3.87 mg·mL-1,and the entrapment efficiency of docetaxel liposomes was 99.26%.Conclusion The content and entrapment efficiency of docetaxel liposomes can be determined conveniently and quickly with ultrafiltration-HPLC.