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Determination of the Content and Entrapment Efficiency of Paclitaxel in Paclitaxel Liposomes by RP-HPLC

Zhu Mei

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Abstract

Objective To establish an HPLC method for the determination of content and entrapment efficiency of paclitaxel in paclitaxel liposomes.Methods The analysis was performed on HYPERSIL C18 column(4.6 mm×250 mm,5 μm).The mobile phase consisted of methanol:acetonitrile:water(40:35:25),and the detection wavelength was 227 nm.Results The linear range for paclitaxel was 1.0 ～200.0 μg/mL(r = 0.9996,n=7).The average recovery was 101.51% and RSD was 1.96%.Conclusion This method is simple,accurate,sensitive form easurement of content and entrapment efficiency of paclitaxel in the liposomes.

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What this paper is about

Objective To establish an HPLC method for the determination of content and entrapment efficiency of paclitaxel in paclitaxel liposomes.Methods The analysis was performed on HYPERSIL C18 column(4.6 mm×250 mm,5 μm).The mobile phase consisted of methanol:acetonitrile:water(40:35:25),and the detection wavelength was 227 nm.Results The linear range for paclitaxel was 1.0 ～200.0 μg/mL(r = 0.9996,n=7).The average recovery was 101.51% and RSD was 1.96%.Conclusion This method is simple,accurate,sensitive form easurement of content and entrapment efficiency of paclitaxel in the liposomes.

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Available abstract

Objective To establish an HPLC method for the determination of content and entrapment efficiency of paclitaxel in paclitaxel liposomes.Methods The analysis was performed on HYPERSIL C18 column(4.6 mm×250 mm,5 μm).The mobile phase consisted of methanol:acetonitrile:water(40:35:25),and the detection wavelength was 227 nm.Results The linear range for paclitaxel was 1.0 ～200.0 μg/mL(r = 0.9996,n=7).The average recovery was 101.51% and RSD was 1.96%.Conclusion This method is simple,accurate,sensitive form easurement of content and entrapment efficiency of paclitaxel in the liposomes.

Key concepts: Paclitaxel, Entrapment, Chromatography, Liposome, High-performance liquid chromatography, Chemistry, Acetonitrile, Linear range

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