Filtration by Microspore Film for Determination of the Entrapment Efficiency of Docetaxel Liposomes

OBJECTIVE To establish a method for determination of the entrapment efficiency of docetaxel liposomes.METHODS HPLC was established for the assay of docetaxel.Lichrospher-RP C18 column(150 mm×6.0 mm,5 μm) was used,with acetonitrile-water(60∶40) as mobile phase,the flow rate was 1.0 mL·min-1,the detection wavelength was 231 nm,and the column temperature was 35 ℃.The 0.22 μm microspore film was used for the separation of docetaxel liposomes and free docetaxel,the feasibility of the method was evaluated.RESULTS Docetaxel could be separated well,the adjuvants did not interfere the determination of docetaxel,and all the concerning indexes according to the requests under such HPLC condition.The free docetaxel was obviously blocked by 0.22 μm microspore film;the method was effective for the separation of docetaxel liposome and free docetaxel.CONCLUSION The method is very suitable for determination of the entrapment efficiency of docetaxel liposomes.