Determination of mycophenolic acid in plasma of kidney transplantation patients by HPLC and its pharmacokinetic

AIM: To determine mycophenolic acid (MPA) in plasma of kidney transplantation patients by HPLC and its pharmacokinetics. METHODS: Discover C18(250.0 mm× 4.6 mm, 5 μm)spectrum analytical column was used and phosphate buffer acetonitrile(45∶55) was adopted as the mobile phase. Flow rate was 0.8 mL/min, detection wavelength was 250 nm, column temperature was 30℃ and the dosage of MPA was 100 μL. The pharmacokinetic parameters of MPA taken by 9 kidney transplantation patients were standard curve calculated in plasma. RESULTS: The standard curve was linear ( r =0.9997) with the range of 0.0995- 27.8516 mg/L for MPA. The limit of detection in plasma was 5 μg/L. The average recovery were 96.30%, 97.82% and 98.62%, respectively. The inter day precision and intra day precision were 3.6%,1.8%, 0.5% and 5.6% , 3.2%, 1.3%, respectively. The results showed that the pharmacokinetic of MPA was in accordance with the one compartment open model. Tmax, Cmax and AUC (0-12 h) was 0.93 h,10.24 mg/L and 31.23 mg/(h· L), respectively. CONCLUSION: The method is simple, quick and precise, and can be used clinically in mentoring the plasma MPA in organ transplantation patients.