STUDY ON THE DRUG-RESISTANCE OF ENTEROBACTER CLOACAE PRODUCING AMPC AND NON PRODUCING AMPC ENZYME

Objective:To investigate the drug-resistance and distribution of 158 strai ns Enterobacter cloacae.Methods:Drug-resistance test to st rain s Enterobacter cloacae was detected in 16 kinds of antibiotics.The drug sens itiv ity tests was performed by the method of Kirby-Bauer paper-diffusion with the s tandard of NCCLS.AmpC enzyme was examined by cefoxitin three dimension test and PCR Amplification of ampC structure gene were studied.Results: The main sources of specimen were sputum,urine and wound secretion.The respirat ory tract was a major attribute of the development of Enterobacter cloacae. Enterobacter cloacae strain emerged mostly in the intensive care unites.The drug resi stance to cefoxitin,cefotaxime and ceftriaxone were high.Results: Of PCR amplification revealed that of 158 Enterobacter cloacae strains,33 strains had ampC structure gene which account for 20.9%.The drug resistance of Enterobacter cloacae p roducing AmpC enzyme were significantly higher than those of non-producing AmpC enzyme.Conclusions:The drug-resistance of Enterobacter clo acae was associated with AmpC enzyme.The best choice of treatment was imipenem .