Analysis gene of strains of enterobacter cloacae producing AmpC β-lactamase and its resistance

To investigate the resistance among clinical isolates of Enterobacter cloacae from Kunming and the relationship between drug resistance and AmpC structure gene and AmpD regulative gene. K-B tests were performed to detect the susceptibility of antimicrobial agents, AmpC β-lactamase in Enterobacter cloacae was detected by three-dimensional test. AmpC and AmpD genes were detected by PCR. AmpC β-lactamase was found 22.3% in 74 strains of Enterobacter cloacae by three-dimensional test. The sensitive rates of drug to the strains producing AmpC β-lactamase were lower than those of strains not producing AmpC β-lactamase. AmpC gene was positive in 66 of 74 tested isolates (89.2%) and AmpD gene was positive in 64 of 74 tested isolates (86.5%) by three-dimensional test. The resistant status of Enterobacter cloacae from Kunming is in a severe situation. The structure gene of AmpC and regulative gene of AmpD of Enterobacter cloacae may be associated with the drug resistance.