Leflunomide on diabetic nephropathy VEGF expression in rat kidney

Objective:To study the fluoride mitter of diabetic nephropathy rat kidney VEGF expression.Methods:Fortyeight male Wistar rats of clean grade were randomly divided into normal control group(A group),model control group(B group),leflunomide group(C group) and losartan group(D group).Diabetes into the mold,respectively,after 8 weeks,12 weeks were observed at 24 h urinary protein excretion,blood urea nitrogen(BUN),creatinine(Scr),renal histopathological changes in renal tissue immunofluorescence detection of VEGF protein expression,RT-PCR used to detect VEGF mRNA expres sion in kidney tissue.Results:8 weeks,12 week diabetic rats(B,C,D groups) Scr,BUN,and 24 h urinary protein compared with normal control group(A group) were significantly higher(P0.01);12 weekend B,C,D rats in Scr,BUN,and 24 h urinary protein than those of 8 weeks,increased;leflunomide(LEF) in the intervention group and the losartan intervention group were lower than the above-mentioned indicators B group,the difference was significant(P0.01),but the LEF group and losartan intervention in the intervention group was no significant difference.Conclusion:Losartan both leflunomide and renal damage in diabetic rats has a protective effect,the mechanism may be achieved by increasing the expression of VEGF.