Study of treatment effect of human umbilical cord blood mesenchymal stem cell transplantation for acute liver injury in rats

Aim:To explore treatment effect of human umbilical cord blood mesenchymal stem cells transplanted to a rat model of acute liver injury.Methods: Human umbilical cord blood mesenchymal stem cells were cultured in vitro.60 SD rats were randomly divided into 3 groups averagely: control group、model group,and treatment +model group.Model group rats were injected intraperitoneally with 50% olive oil solution of carbon tetrachloride to induce acute liver injury,To evaluate modeling results,ALT / AST levels in serum and liver biopsy were analyzed post-model.Rats of treatment +model group were treated with human umbilical cord blood mesenchymal stem cell suspension(3×106-5×106 / only) through local multi-point injection into the liver,then the rats were induced into acute liver injury disease models with 50% olive oil solution of carbon tetrachloride 24h post-transplantation.Survival of experimental animals and liver function improvement were analyzed post-transplantation,so does differentiation of transplanted cells in the liver.Results: Human umbilical cord blood cells cultured in vitro accord with the general biological properties of mesenchymal stem cells.There was statistically significant difference between model group and treatment +model group(P0.05).There were statistically significant difference about AST/ALT levels between two groups as well(P0.05).Transplanted cells in animal liver had a differentiation tendency to human liver-like cells.Conclusion:Transplantation of human umbilical cord blood mesenchymal stem cells could improve the survival rate of the model of acute liver injury.