Study on the self-differentation potential of human umbilical cord blood mesenchymal stem cells to liver-like cellsin vivo in rats models suffered from acute hepatic necrosis(AHN)

Objective To explore the self-differentiation potential of human umbilical cord blood mesenchymal stem cells to liver-like cells in vivo in rats models suffered from acute hepatic necrosis(AHN).Methods hUCBMSCs were injected into the liver of rats in which AHN was induced by carbon tetrachloride(CCl4)24 h post-molding.AHN rats model was scarified from 1 to 4 weeks post-molding.The transdifferentiation status of the hUCBMSCs was evaluated using immunohistochemistry and real-time RT-PCR.Results The cell line cultured in vitro was in accord with the biological characteristics of human umbilical cord blood mesenchymal stem cell.Immunohistochemistry and real-time PCR analysis showed that transplanted hUCBMSCs expressed several human liver tissue-specific markers(ALB、AFP、CK19、CK18、HepPar)in ratswith AHN.Amplified sequences were corresponding to the same sequence in GENEBANK.Conclusion Human umbilical cord blood mesenchymal stem cells have self-differentiation potential to liver-like cells in vivo in rats models suffered from AHN.