Effect of gadolinium chloride on liver ischemia reperfusion injury in rats after administration of lipopolysaccharide

Objective To evaluate the effect of Kupffer cells blockade on liver ischemia reperfusion injury in rats.Methods Twenty-four male Wistar rats were randomly divided into 4 groups.Control group(Ⅰ) received a laparotomy without additional operation.Control+lipopolysaccharide(LPS) group(Ⅱ) received an injection of LPS after laparotomy.I/R+ LPS group(Ⅲ) received an infusion of acidic 0.9%NaCl solution in a comparable volume before operation of ischemia and reperfusion,and GdCl_3+I/R+ LPS group(Ⅳ) were pretreated with 0.5% GdCl_3(pH 3.5) in a dosage of 10 mg/kg.A laparotomy was performed after GdCl_3 was infused into the tail vein at 24 and 48 h.An atraumatic clip was placed across the portal vein,hepatic artery,and bile duct just above the branch of right lateral lobe.After ischemia for 45 min,the peritoneal cavity was sutured,and animals were allowed to recover and to reperfuse for 1 h.Then,LPS were injected into their peritoneal cavity.Following this period,serum and tissue were collected from each animal and frozen at-70 ℃ for subsequent liver enzyme and cytokine determination,histopathological assessment,MDA,GSH and TNF-α contents in the liver.Results After ischemia for 45 min and reperfusion for 1 h,serum level of ALT,contents of MDA,GSH and TNF-α in the liver tissue of group Ⅳ were significantly decreased(P0.001),while GSH in the liver tissue of GdCl_3+ LPS group was significantly increased(P0.05).Conclusion Pretreatment with GdCl_3 has a markedly and significantly protective effect on liver ischemia-reperfusion injury in rats after adiministration of LPS.