Optimization of Ultrasonic-assisted Extraction of Polysaccharides from Enteromorpha prolifera by Response Surface Methodology

We report the use of response surface methodology for the optimization of process conditions for the ultrasonic-assisted hot water extraction of polysaccharides from Enteromorpha prolifera. The effects of three independent variables (namely, extraction temperature, length of extraction and ratio of water to material) on the yield of polysaccharides from E. prolifera (PEP) were evaluated. Extraction temperature of 80 ℃, length of extraction of 28 min and ratio of water to material of 63:1 (mL/g) were found optimum. Under these optimized conditions, the experimental value of PEP yield was 25.84 mg/g, in good agreement with the predicted value. Therefore, the optimized process is very reliable.