Effects of Fosinopril on Laminin and mRNA Expression of Cultured Glomerular Mesangial Cell of Rat

Objective To observe the effect of Fosinopril (FOS),the new generation angiotensin-converting enzyme inhibitor (ACEI),on expression of Laminin (LN),a factor of extracellular matrix (ECM),by rat glomerular mesangial cell (GMC) cultured in vitro; to investigate the mechanism of FOS on prevention and treatment of glomerular sclerosis.Methods Rat GMCs were cultured in classic way. The cultured cell were divided into 3 groups (1) Control group,(2) LPS group,and (3) LPS+FOS group. LN concentration in GMC supernatant fluid was detected by ELISA; and LN mRNA expression was estimated by semiquantitative real-time RT PCR.Results LN secretion and mRNA expressions were obviously higher in LPS group than those in control group(P 0.05) and decreased distinctively in LPS+FOS group compared with LPS group(P 0.05) .Conclusion FOS has obvious inhibited LN expression of rat GMC at both protein level and mRNA level,which suggest that the inhibition by FOS of components of glomerular extracellular matrix produced by rat glomerular mesangial cells is one of important mechanisms for prevention the development of glomerulosclerosis.