Effects of fosinopril on β_1-integrin expression and extracellular matrix secretion of rat glomerular mesangial cell

Objective To observe the effects of fosinopril(FOS)on β1-integrin expression and extracellular matrix(EMA)secretion in lipopolysaccharide(LPS)-induced rat glomerular mesangial cells(GMC).Methods GMC of rats were cultured in vitro.The cells at passages 3 to 10 were used in the experiment after identification.The experiment included five groups:control group,LPS group,groups with high,middle and low dose of FOS(FOS1,FOS2 and FOS3 group).The supernatant were collected 6 h,12 h,and 24 h after the cells culture.The ECM,including laminin(LN),fibronectin(FN)and type Ⅳ collagen(Col Ⅳ)was detected by the enzyme-linked immunosorbent-assay(ELISA).The expression of β1-integrin mRNA was detected by semi-quantitative real-time RT-PCR.Results Cultured GMC of rats can secrete certain amount of ECM in vitro.At each time point,the ECM secretion was significantly higher in LPS group than that in control group(P 0.01),but significantly lower in all FOS groups than that in LPS group(P 0.01).Cultured GMC of rats can express low level of β1-integrin mRNA in vitro.At each time point,β1-integrin mRNA expression was significantly higher in LPS group than that in control group,but was significantly lower in FOS groups than that in LPS group.Conclusions LPS can induce the GMC β1-integrin mRNA expression and ECM secretion in rats.Meanwhile,FOS can inhibit the GMC β1-integrin mRNA expression and ECM secretion in rats in dose-dependent manner.FOS can protect the kidneys from non-hemodynamic mechanism by inhibiting cytokine and ECM secretion