Effects of Fosinopril on Transforming Growth Factor-Beta 1 Secretion and mRNA Expression of Cultured Rats Glomerular Mesangial Cell

Objective To observe the effects of fosinopril(FOS),a new generation angiotensin-converting enzyme inhibitor(ACEI),on protein and mRNA expression of transforming growth factor-β_1(TGF-β_1) of rat glomerular mesangial cell(GMC) induced by lipopolysaccharide(LPS);to demonstrate the preventive mechanism against glomerular sclerosis by applying FOS.Methods The cultured GMC in classic way were divided into 3 groups:control group;LPS group;LPS+FOS group.TGF-β_1 concentration in GMC supernatant fluid was detected by ELISA;TGF-β_1 mRNA expression was determined by semiquantitative real-time RT-PCR.Results LPS group was obviously higher than control groups in TGF-β_1 secretion and mRNA expression,while LPS+FOS group decreased distinctively in TGF-β_1 secretion and mRNA expression compared with LPS group.Conclusions FOS has obviously inhibited on TGF-β_1 expression of rat GMC both at protein level and mRNA level,which reveals that it may be an important mechanism by FOS on restraining the development of glomerulosclerosis.