Effects of cryotherapy on VEGF,Ki67 protein expression in prostate cancer of mice

Objective:To explore the effects of cryotherapy on the angiogenesis and cell proliferation in prostate cancer of mice. Methods: Mouse experimental models with prostate cancer were established. Experimental mice were randomly divided into control group and freezing group. Ki67,VEGF protein expression were detected by immunohistochemical staining at different time after the freezing. Results: There were no change in Ki67,VEGF protein expressions at different time in control group (P0.05),Ki67, VEGF protein expressions in freezing group were decreased.The lowest value was detected at the third day after freezing,the value increased slowly after that (P0.01).There were significant changes in VEGF,Ki67 protein expressions at different time in freezing group(P0.05).Correlation analysis showed that VEGF protein expression was positively correlated with Ki67(r=0.6921,P0.01). Conclusion: Cryotherapy can considerably reduce VEGF and Ki67 protein expression,thus inhibiting the growth of prostate cancer in mice.