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2008•Chinese Journal of Hospital PharmacyRequires access

The reversing effect of nimesulide on multi-drug resistance of breast cancer cell line MCF-7/ADM

Yan Li

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Abstract

OBJECTIVE To evaluate the effect of nimesulide on breast cancer cell line MCF-7/S and MCF-7/ADM and explore the mechanism of nimesulide reversing multi-drug resistance of breast cancer cell line MCF-7/ADM.METHODS Cell Suppression rates were treated by MTT assay.Cell Rh123 concentration and P-170、GST-π expressions were detected with flow cytometry.RESULTS Nimesulide can inhibit MCF-7/S and MCF-7/ADM cell growth in a dose-and time-dependent manner,and Cell Suppression rates of MCF-7/S was higher than that of MCF-7/ADM at same dose and time.Rh123 fluorescence intensity of MCF-7/ADM cell obviously increased and P-170、GST-π expressions clearly decreased when treated with 120μmol/L Nimesulide.CONCLUSION Nimesulide can partly reverse multi-drug resistance of breast cancer cell line MCF-7/ADM by decrease of P-170 and GST-π expressions.

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OBJECTIVE To evaluate the effect of nimesulide on breast cancer cell line MCF-7/S and MCF-7/ADM and explore the mechanism of nimesulide reversing multi-drug resistance of breast cancer cell line MCF-7/ADM.METHODS Cell Suppression rates were treated by MTT assay.Cell Rh123 concentration and P-170、GST-π expressions were detected with flow cytometry.RESULTS Nimesulide can inhibit MCF-7/S and MCF-7/ADM cell growth in a dose-and time-dependent manner,and Cell Suppression rates of MCF-7/S was higher than that of MCF-7/ADM at same dose and time.Rh123 fluorescence intensity of MCF-7/ADM cell obviously increased and P-170、GST-π expressions clearly decreased when treated with 120μmol/L Nimesulide.CONCLUSION Nimesulide can partly reverse multi-drug resistance of breast cancer cell line MCF-7/ADM by decrease of P-170 and GST-π expressions.

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Available abstract

OBJECTIVE To evaluate the effect of nimesulide on breast cancer cell line MCF-7/S and MCF-7/ADM and explore the mechanism of nimesulide reversing multi-drug resistance of breast cancer cell line MCF-7/ADM.METHODS Cell Suppression rates were treated by MTT assay.Cell Rh123 concentration and P-170、GST-π expressions were detected with flow cytometry.RESULTS Nimesulide can inhibit MCF-7/S and MCF-7/ADM cell growth in a dose-and time-dependent manner,and Cell Suppression rates of MCF-7/S was higher than that of MCF-7/ADM at same dose and time.Rh123 fluorescence intensity of MCF-7/ADM cell obviously increased and P-170、GST-π expressions clearly decreased when treated with 120μmol/L Nimesulide.CONCLUSION Nimesulide can partly reverse multi-drug resistance of breast cancer cell line MCF-7/ADM by decrease of P-170 and GST-π expressions.

Key concepts: Nimesulide, MCF-7, Flow cytometry, Reversing, Chemistry, Cell culture, Cell growth, Pharmacology

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