EXPERIMENTAL STUDY ON THE REVERSAL EFFECT OF HEATING COMBINED WITH 5-BROMOTETRANDRINE(W198)ON MUTI-DRUG RESISTANT OF BREAST CANCER CELL LINE MCF-7/ADM

[Objective]To observe the reversal effect of heating combined with 5-bromotetrandrine(W198)on muti-drug resistant of breast cancer cell line MCF-7/Adm.[Methods]The breast cancer cell line MCF-7 was included as control,and drug resistant the breast cancer line MCF-7/Adm as participants.At different temperature at the range of 37℃～42℃ and different concentrations of W198 at the range of 0.125 μmol/L～1.0 μmol/L,the muti-drug resistant breast cancer cell line MCF-7/Adm was treated with 1 μg·ml﹣1 Adm for one hour.The inhibitory rates of cell growth for MCF-7 and MCF-7/Adm,fluorescence intensity of Adm in cells and cell surface P-glycoprotein(p-gp)were detected,their association of variance tendency with the temperature of heating and concentration of W198 were analyzed.[Results]The sensitivity of MCF-7/Adm to Adm was significantly lower than that of MCF-7.With the increased temperature of heating and concentration of W198,the inhibitory rate and intracellular concentration of Adm of MCF-7/Adm cells increased,while expression of p-gp decreased.1.0μmol·L-1 W198 combined with 1μg·ml-1 Adm at 42℃ could greatly reverse the multi-drug resistance of MCF-7/Adm to the Adm.[Conclusion]W198 combined with heating showed joint action to the multi-drug resistance of MCF-7/Adm cells.