Analysis of general biological characteristics of human breast cancer drug resistance cell line MCF-7 /ADM

Objective To establish breast cancer drug resistance cell line MCF-7 /ADM and analyze its biologic features. Methods A new breast cancer drug resistant cell line MCF-7 /ADM was established by stepwise increasing concentration of adriamycin. Cell growth curve and doubling time change were analyzed by MTT method. Colony-forming capacity was tested by colony formation assay,and immuno-cytochemistry staining was adopted to analyze the biological characteristics of the new breast cancer drug resistant cell line MCF-7 / ADM. Results The breast cancer adriamycin-resistant cell line MCF-7 / ADM was successfully induced. The resistance index was 9. 1. Comparing with the parental ADM-sensitive cells,the resistance index of multi-drug resistant cell line MCF-7 / ADM was kept above 7. 0,showing stable drug resistance,shorter withdrawal culture doubling time,and significant difference in plate colony count,volume of colony( P 0. 01). Immunocytochemical staining analysis showed that the positive expression rates of HER-2 and ALDH1 protein were higher in multi-drug resistant cell line MCF-7 / ADM than those in parental MCF-7 cells( P 0. 05),and ER protein expression was obviously lost compared to parental MCF-7 cells. Conclusion The cell line MCF-7 / ADM established in this study has basic biological characteristics of multidrug-resistant cells,indicating increasing independent growth capacity and self-proliferation capacity in vitro,obvious dedifferentiation trend,poor curative effect on endocrinotherapy,and the worsening condition of breast cancer.