Simultaneous Determination of Ursolic Acid and Oleanolic Acid in Herba Plantaginis by RP-HPLC

Ursolic acid and oleanolic acid were extracted from herba plantaginis by ultrasonic-microwave synergistic extraction technology,and a method for fast simultaneous the determination the contents of ursolic acid and oleanolic acid in herba plantaginis was established by reversed phase-high performance liquid chromatography(RP-HPLC).The Agilent C18 column(4.6mm×250mm,5μm)was used with acetonitrile-0.5% ammonium acetate solution(80∶20,V/V)as the mobile phase at the flow rate of 0.8mL/min,and UV detection wavelength was at 210nm.The baselines of ursolic acid and oleanolic acid separated in 12 min under these chromatographic conditions.The calibration curves of ursolic acid and oleanolic acid were linear in the range of 1.01—10.1μg(r=0.9995) and 0.39—3.9μg(r=0.9992),respectively.The average recoveries were 98.8%and 97.9%,and the RSD were 1.7%(n=5)and 1.3%(n=5),respectively.The method is simple,quick and accurate,that can be used for simultaneous the determination of the contents of oleanolic acid and ursolic acid in herba plantaginis.