Study on expression of recombinant plasmid PcDNA3-AMG for human amelogenin in COS-1 cell line

Objective To investigate the amelogenin expression of recombinant plasmid PcDNA3-AMG in COS-1 cell line.Methods PcDNA3-AMG was used to transfect COS-1 cells firstly.An appropriate concentration of Zeoin was chosen for the selection of stably transfected cell colonies.The ELISA technique was applied to detect the expression of recombinant amelogenin.Results The amelogenin was detected in the stably transfected COS-1 cells in both cell culture supernatant and cell homogenate,and the concentration of expressed amelogenin in PcDNA3-AMG transfected cell homogenate was as high as 0.253 μg/ml.Conclusions The results provided basis for the preparation of recombinant AMG by PcDNA3-AMG transfected eukaryrotic cell line COS-1.