2005•Journal of Dental Prevention and TreatmentRequires access

Study on Expression of Recombinant Plasmid PsecTaq2A-AMG for Human Amelogenin in COS-1 Cell Line

Jincai Zhang

Abstract

Objective To investigate the amelogenin expression of recombinant plasmids PsecTaq2A-AMG in COS1-cell line. Methods PsecTaq2A-AMG was used to transfect COS-1 cells firstly. Appropriate concentration of Zeoin was chosen for the selection of stably transfected cell colonies. The immunohistochemistry and ELISA techniques were applied to detect the expression of recombinant amelogenin. Results The amelogenin was detected in the stably trasfected COS-1 cells in both cell culture supernatant and cell homogenate, the concentration of expressed amelogenin in PsecTaq2A-AMG transfected cell homogenate is as high as 0.877 μg/ml. Conclusion The results thus provided bases for the preparation of recombinant amelogenin by PsecTaq2A-AMG transfected eukaryotic cell line COS-1.

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Key concepts: Amelogenin, Transfection, Recombinant DNA, Molecular biology, Plasmid, Cell culture, Cell, Chemistry