2006•Journal of Dental Prevention and TreatmentRequires access

Expression of the Recombinant Plasmid PcDNA3.1/sTNFRI in CHO Cells

Chenrong Xu

Abstract

Objective To detect the expression of recombinant plasmid PcDNA3.1/sTNFRⅠin vitro.Methods CHO cells were transfected with recombinant plasmid PcDNA3.1/sTNFRⅠ by liposome.sTNFRⅠexpressed in cell culture supernatant was detected by ELISA.Results It was much higher of sTNFRⅠin transfected cell culture supernatant than control groups.Conclusion These results showed that the recombinant plasmid PcDNA3.1/sTNFRⅠcan be expressed in mammalian cells.

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Key concepts: Recombinant DNA, Plasmid, Transfection, Chinese hamster ovary cell, Molecular biology, Cell culture, In vitro, Liposome