ScholarLens Back to search
2006•Anhui nongye kexueRequires access

Determination of Content of Bergenin in Bergenrae pacumbis by RP-HPLG

Zhao Su-qin

Open publisher page 0 citations

Abstract

Objective: To establish a quantitative method of determination of bergenin in Bergenrae pacumbis by RP-HPLC.Method: The sample were separated on the column of Zorbax SB-C_(18)(250 mm×4.6 mm,5 μm) which eluted with methanol and water(25∶75).Detected wavelength at 274 nm,flow rate at 0.8 ml/min,column temperature at 35 ℃;The Supersonic extraction of bergenin was studied by orthogonal experiments.Results: Bergenin was base-isolated.The linear ranges of bergenin was 0.306～2.14 μg(r=0.999?8);The average recoveries were 101%(RSD=2.2%).Conclusions: The method is rapid and precise.

About this research paper

What this paper is about

Objective: To establish a quantitative method of determination of bergenin in Bergenrae pacumbis by RP-HPLC.Method: The sample were separated on the column of Zorbax SB-C_(18)(250 mm×4.6 mm,5 μm) which eluted with methanol and water(25∶75).Detected wavelength at 274 nm,flow rate at 0.8 ml/min,column temperature at 35 ℃;The Supersonic extraction of bergenin was studied by orthogonal experiments.Results: Bergenin was base-isolated.The linear ranges of bergenin was 0.306～2.14 μg(r=0.999?8);The average recoveries were 101%(RSD=2.2%).Conclusions: The method is rapid and precise.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective: To establish a quantitative method of determination of bergenin in Bergenrae pacumbis by RP-HPLC.Method: The sample were separated on the column of Zorbax SB-C_(18)(250 mm×4.6 mm,5 μm) which eluted with methanol and water(25∶75).Detected wavelength at 274 nm,flow rate at 0.8 ml/min,column temperature at 35 ℃;The Supersonic extraction of bergenin was studied by orthogonal experiments.Results: Bergenin was base-isolated.The linear ranges of bergenin was 0.306～2.14 μg(r=0.999?8);The average recoveries were 101%(RSD=2.2%).Conclusions: The method is rapid and precise.

Key concepts: Bergenin, Chromatography, Chemistry, Elution, High-performance liquid chromatography, Methanol, Extraction (chemistry), Organic chemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of Content of Bergenin in Bergenrae pacumbis by RP-HPLG — Research Paper | ScholarLens