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2010•Zhongguo shiyan fangjixue zazhiRequires access

Determination of Bergenin in Compound Fufangaidicha Tablet by HPLC

Wenli Li

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Abstract

Objective:To establish a HPLC method for determination of bergenin in compound Fufangaidicha Tablet.Method:Bergenin was determined by HPLC.Agilent DB-Cl8 column(4.6 mm ×250 mm,5 μm)was used.The mobile phase consisted of methanol-water(20∶ 80).The flow rate was 1.0 mL·min-1.The column temperature was at 35 ℃;The detection wavelength was at 275 nm.Result: The linear ranges of Bergenin was in 0.050 72 ～0.760 8 μg(r =0.999 9),the average recovery(n =9) was 100.6% with RSD 1.8%.Conclusion:The method can be used for the quality control of Fufangaidicha Tablets.

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Objective:To establish a HPLC method for determination of bergenin in compound Fufangaidicha Tablet.Method:Bergenin was determined by HPLC.Agilent DB-Cl8 column(4.6 mm ×250 mm,5 μm)was used.The mobile phase consisted of methanol-water(20∶ 80).The flow rate was 1.0 mL·min-1.The column temperature was at 35 ℃;The detection wavelength was at 275 nm.Result: The linear ranges of Bergenin was in 0.050 72 ～0.760 8 μg(r =0.999 9),the average recovery(n =9) was 100.6% with RSD 1.8%.Conclusion:The method can be used for the quality control of Fufangaidicha Tablets.

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Available abstract

Objective:To establish a HPLC method for determination of bergenin in compound Fufangaidicha Tablet.Method:Bergenin was determined by HPLC.Agilent DB-Cl8 column(4.6 mm ×250 mm,5 μm)was used.The mobile phase consisted of methanol-water(20∶ 80).The flow rate was 1.0 mL·min-1.The column temperature was at 35 ℃;The detection wavelength was at 275 nm.Result: The linear ranges of Bergenin was in 0.050 72 ～0.760 8 μg(r =0.999 9),the average recovery(n =9) was 100.6% with RSD 1.8%.Conclusion:The method can be used for the quality control of Fufangaidicha Tablets.

Key concepts: Bergenin, Chromatography, High-performance liquid chromatography, Chemistry, Methanol, Organic chemistry

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