Determination of Caffeic Acid,Ferulic Acid,Rosmarinic Acid in Rosmarinus officinalis by HPLC with Changing Ultraviolet-visible Wavelength

Objective:To establish the method for determining the content of caffeic acid,ferulic acid,ros-marinic acid in Rosmarinus officinalis for its quality standards to provide the scientific basis for the study.Caffeic acid,ferulic acid,rosmarinic acid were determined by HPLC simultaneously with changing ultraviolet-visible wave-length.Chromatographic condition was composed of C18(4.6 mm × 250 mm,5 μm),mobile phase methanol – 0.1% phosphoric acid(32:68);Flow rate was 1.0 mL·min-1;the detection wavelength of caffeic acid at 323 nm,Ferulic acid at 316 nm and rosmarinic acid at 329 nm.the isolation effect among caffeic acid,ferulic acid,and ros-marinic acid showed good linear correlation,the average recoveries were 103.7%,99.5%,101.7%;RSD were 1.5%,1.2%,1.5%.the method and was convenient,accurate and good reappearance,It can be used as quantity basis of the quality control of R.officinalis.