2008Chinese Journal of Laboratory DiagnosisRequires access

Study on Hela cell apoptosis induced by 20(s)-ginsendoside Rg3 in cervical cancer

Xuexia Liu

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Abstract

Objective To study effect of the 20(s)-ginsendoside Rg3(SPG-Rg3) induced apoptosis on human cervical cancer line Hela cell.Methods The growth and apoptosis of Hela cell was detected by MTT assay,flow cytometry and AO/EB fluorescence double-dry technology.Results SPG-Rg3 could inhibit the growth of Hela cell(P0.05),the percentage of cells in S stage was increased,the percentage of cells in G2/M stage was decreased(P0.01),and the cells of apoptosis was add.Conclusion SPG-Rg3 could induce Hela cell apoptosis,and may be used as new method for cervical cancer.

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What this paper is about

Objective To study effect of the 20(s)-ginsendoside Rg3(SPG-Rg3) induced apoptosis on human cervical cancer line Hela cell.Methods The growth and apoptosis of Hela cell was detected by MTT assay,flow cytometry and AO/EB fluorescence double-dry technology.Results SPG-Rg3 could inhibit the growth of Hela cell(P0.05),the percentage of cells in S stage was increased,the percentage of cells in G2/M stage was decreased(P0.01),and the cells of apoptosis was add.Conclusion SPG-Rg3 could induce Hela cell apoptosis,and may be used as new method for cervical cancer.

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Available abstract

Objective To study effect of the 20(s)-ginsendoside Rg3(SPG-Rg3) induced apoptosis on human cervical cancer line Hela cell.Methods The growth and apoptosis of Hela cell was detected by MTT assay,flow cytometry and AO/EB fluorescence double-dry technology.Results SPG-Rg3 could inhibit the growth of Hela cell(P0.05),the percentage of cells in S stage was increased,the percentage of cells in G2/M stage was decreased(P0.01),and the cells of apoptosis was add.Conclusion SPG-Rg3 could induce Hela cell apoptosis,and may be used as new method for cervical cancer.

Key concepts: HeLa, Apoptosis, Flow cytometry, MTT assay, Cell, Chemistry, Cervical carcinoma, Cervical cancer

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