2010•Journal of Hunan Normal UniversityRequires access

Induction of Apoptosis in Human Cervical Cancer HeLa Cells by Casticin

Sheng Xi

Abstract

Objective To investigate the effects of casticin(CAS) on apoptosis of human cervical cancer HeLa cell line.Methods Human cervical cancer HeLa cells were cultured in vitro.Histone/DNA fragments in medium were determined using ELISA assay.The percentage of the sub-G1 population was determined by flow cytometry of PI fluorescence staining.Caspase-3 activity in HeLa cells was examined using ELISA assay.Results CAS significantly increased leakage of the histone/DNA fragments in HeLa cell line(P0.05),in a concentration-dependent manner.Meanwhile,CAS significantly promoted the increase of the percentage of the sub-G1 cell population in human cervical cancer HeLa cells(P0.05).CAS effectively activated Caspase-3 in human cervical cancer HeLa cells(P 0.05),in a concentration-dependent manner.Conclusion CAS can effectively induce apoptosis of human cervical cancer HeLa cells.

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Key concepts: HeLa, Apoptosis, Flow cytometry, Molecular biology, Population, Cell culture, Cell, Cervical cancer