Construction and sequence analyzing of the recombinant plasmid affecting gene CXCR4 translation by RNA jnterfering

Objective To construct the recombinant plasmid carrying shRNA to CXCR4 and analyze the nucleic acid sequence for further searching new gene therapy method of tumor.Methods Two DNA sequences containing small hairpin structure were designed and synthesized.The complement form was obtained by annealing and inserted into vector Psilencer2.1-U6 neo,and the recombinant plasmid was transformed into DH5a strain.Finally the plasmid identified by restriction enzyme Was used for sequence analysis.Results The recombinant psilencer2.1-U6 neo carrying shRNA to CXCR4 had been constructed and the aim sequence had been obtained.Conclusion The construction of the recombinant plasmid carrying shRNA to CXCR4 lays the basis for the study of its inhibitive effect on tumor.