Expression of MCM4 and p53 and their clinical significance in cervical intraepithelial neoplasia and carcinoma in cervix

Purpose To investigate the MCM4 and p53 expression and their significance in cervical intraepithelial neoplasia (CIN) and cervical carcinoma.Methods MCM4 and p53 expression was examined by immunohistochemistry (EliVision method) in 48 cases of cervical carcinoma,15 cases of CIN 1,27 cases of CIN 2~3 and 15 cases of normal cervical epithelial tissues.Results The positive expression rates of MCM4 and p53 in normal cervical epithelium,CIN 1,CIN 2~3 and cervical carcinoma were 6.67%,20.00%,55.56% and 83.33%,and 0,6.67%,18.52% and 41.67%,respectively.The positive expression rate of MCM4 was significantly different between CIN and cervical carcinoma (P0.05).MCM4 expression was closely correlated with pathological grade of cervical carcinoma and clinical stage (P0.05).However,there was no significant association between MCM4 expression and p53 in cervical carcinoma and CIN (P0.05).Conclusions MCM4 is useful for evaluating cell proliferative activity.Potential applications of it include the identification and diagnosis of malignancy and precancerous lesions,assessment of prognosis and assistance of clinical management.