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Cloning and sequencing of PGIP gene in Prunus salicina

Guangping Li, Qiao Yushan, Tao Jian-min, Zhihong Gao, Zhen Zhang

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Abstract

A target fragment with a full length of 1 192 bp(Genbank accession:DQ200847) was amplified with genomic DNA of Prunus salicina leaves as the templates and the conservative sequences of PGIP gene as the primers.Its sequencing showed that it was 96%～99% identical with the sequences of PGIP genes in Prunus armeniaca,P.persica,P.mahaleb,P.mume and contained one complete open reading frame and one intron,and the sequence that it encoded was a typical lecucine-repeated sequence;in its evolution tree,this sequence of PGIP gene was in the same group as the sequences of PGIP gene in P.armeniaca,P.persica,P.mahaleb,P.mume was in.as a result,a gene resource was provided for molecular breeding of plants.

About this research paper

What this paper is about

A target fragment with a full length of 1 192 bp(Genbank accession:DQ200847) was amplified with genomic DNA of Prunus salicina leaves as the templates and the conservative sequences of PGIP gene as the primers.Its sequencing showed that it was 96%～99% identical with the sequences of PGIP genes in Prunus armeniaca,P.persica,P.mahaleb,P.mume and contained one complete open reading frame and one intron,and the sequence that it encoded was a typical lecucine-repeated sequence;in its evolution tree,this sequence of PGIP gene was in the same group as the sequences of PGIP gene in P.armeniaca,P.persica,P.mahaleb,P.mume was in.as a result,a gene resource was provided for molecular breeding of plants.

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Available abstract

A target fragment with a full length of 1 192 bp(Genbank accession:DQ200847) was amplified with genomic DNA of Prunus salicina leaves as the templates and the conservative sequences of PGIP gene as the primers.Its sequencing showed that it was 96%～99% identical with the sequences of PGIP genes in Prunus armeniaca,P.persica,P.mahaleb,P.mume and contained one complete open reading frame and one intron,and the sequence that it encoded was a typical lecucine-repeated sequence;in its evolution tree,this sequence of PGIP gene was in the same group as the sequences of PGIP gene in P.armeniaca,P.persica,P.mahaleb,P.mume was in.as a result,a gene resource was provided for molecular breeding of plants.

Key concepts: GenBank, Prunus salicina, Gene, Prunus, Biology, Accession number (library science), Genetics, Intron

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