Cloning and Bioinformatic Analysis of PGIPGene fromPrunus caoyuan

Disease resistance mechanism was studied by methods of cloning of the PGIP gene in Prunus caoyuan.A DNA fragment about 1 kb was amplified from the genomic DNA of Prunus caoyuan leaves by PCR with a pair of specific primers based on the conserved sequences of the PGIP genes of genus Prunus.Sequence analysis showed that the fragment contains a full coding region of 1 193 bp(GenBank accession:GU068977).This sequence had a full open reading frame encoding the PGIP,and eontained two exons interrupted by one intron.The total exons were comprised by 990 bp of deoxynucleotide encoding 330 amino acid.A conserved leucine-rich fragmenthad existed in the derived protein sequence.Sequencing analysis showed that it was 95% to 99% identical with the sequences of Prunus PGIP genes including P.salicina,P.armeniaca,P.persica,P.mahaleb,P.mume.Phylogenic tree showed that genetic relationship within the genus was closer and between the genera was farther.A PGIP gene of Prunus caoyuan was cloned.As a result,a gene resource was provided for molecular breeding of plants.