Positive effect of Crucian carp decoction on the expression of Th17 cells inflammatory factors in rats with nephrosis induced by adriamycin

Objective To observe the therapeutic effect of Crucian carp decoction on rats with nephrosis induced by adriamycin,and explore its mechanism of action.Methods Fourty healthy 8-week old male Wistar rats were randomly divided into crucian carp decoction group,Irbesartan group,nephrosis group,and control group.The rat model of nephrosis was induced by a single tail vein injection of adriamycin 6.2 mg/kg body weight.The rats of the crucian carp decoction group were given oral gavage of crucian carp decoction once everyday for 9 weeks,and urinary protein was checked each week.All rats were killed at week 9.Histological changes were observed by light microscopy and blood biochemical indices were detected.IL-17,IL-23,IL-1β,and CXCR2 expressions in the kidney were assessed by immunohistochemistry,Serum IL-17,IL-23,IL-1β levels were assayed by ELISA.Result Rats in the crucian carp decoction group had a higher level of albumin compared with that of the nephrosis group(P=0.03),significantly lower than that of the control group(P =0.01) and the expressions of IL-17,IL-23,and CXCR2 in the kidney were significantly lower than those in the nephrosis group(P =0.01,P =0.01,P =0.02),significantly higher than those of the control group(P=0.04,P=0.007,P=0.05).Conclusions The increase of Th17 cell inflammatory factors may be one of the pathogenetic mechanisms in rats with adriamycin-induced nephrosis.Crucian carp decoction can improve the albumin level of rats with nephrosis,and alleviate renal impairment induced by IL-17,IL-23 and CXCR2.