2013•Journal of Beijing University of Traditional Chinese MedicineRequires access
Influence of Jianpi Yishen Huayu Tongluo Fang on oxidative stress in rats with diabetic nephropathy
WU Ge-dun
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Abstract
Objective To observe the kidney protection effect and influence of Jianpi Yishen Huayu Tongluo Fang on oxidative stress in rats with diabetic nephropathy(DN) induced by streptozocin(STZ).Methods Male Wistar rats(n=74) were intraperitoneally injected STZ(45 mg/kg) for establishing model,and then divided,after successful modeling and according to body weight,into normal grouop,model group,irbesartan(17.5 mg/kg) group and low-dose(30.8 mg/kg) and high-dose(123.2 mg/kg) Jianpi Yishen Huayu Tongluo Fang groups(low-dose group and high-dose group).The body weight and 24-hour urine protein quantitate were measured every 4 weeks.On the 14th week the rats were executed for recording the ration of kidney weight to body weight(KW/BW) and detecting the levels of serum triglyceride(TG),blood urea nitrogen(BUN),serum creatinine(Scr),nitric oxide(NO),malondialdehyde(MDA) and superoxide dismutase(SOD).The pathological changes and scores of kidney tissue were observed and analyzed after staining of HE,Mallory and PASM-HE.Results In low-dose group and high-dose group,the body weight increased significantly(P0.05),KW/BW decreased significantly,pathological changes of kidney tissue were relieved,levels of serum TG,BUN and Scr decreased significantly(P0.05),activity of serum SOD increased(P0.05) and MDA decreased(P0.05) compared with model group.Conclusion Jianpi Yishen Huayu Tongluo Fang can play a role of protecting kidney through antagonizing oxidative stress in DN rats.