The mechanism of the intervention effect of sivelestat sodium in the treatment of the rats with acute pancreatitis associated lung injury

Objective To explore the effect and its possible mechanism of sivelestat sodium on the lung injury of severe acute pancreatitis SD rats.Methods Twenty-four healthy SD rats were randomly divided into three groups: the control group,the severe acute pancreatitis group and the sivelestat sodium treated group.SAP models were induced by retrograde injection of 5% taurocholate sodium into the biliopancreatic duct of SD rats.In the drug group sivelestat sodium was given on the basis of SAP model.While in the control group and the severe acute pancreatitis group,isometric physiological saline was given.Six hours later after the three groups of model were successfully established,serum neutrophil elastase(NE) activity was determined with the method of enzyme-linked immunosobent assay(ELISA) in the venous.The serum amylase(AMS) was determined with routine method.Wet to dry weight ratio(W/D) of lung and MPO in homogenate of harvested lung were assayed.Expression of NF-κB in pulmonic tissues was observed with immunohistochemistry(IHC).The pathologic change in pulmonic tissues were also measured.Results Compared with the control group,the concentration of MPO,NF-κB and W/D ratio of lung were significantly increased(P0.05) in the severe acute pancreatitis group.And the level of the serum NE was significantly increased(P0.05) in the severe acute pancreatitis group.The AMS was also significantly increased(P0.05) in the severe acute pancreatitis group.There were obviously histopathological injury of pulmonic tissues in the severe acute pancreatitis group(P0.05).Compared with the severe acute pancreatitis group,the concentration of MPO,NF-κB and lung W/D ratio were significantly descended(P0.05) in the sivelestat sodium treated group,and the level of serum NE was markedly lowered(P0.05).But the serum AMS was not significantly decreased(P0.05) in the sivelestat sodium treated group.The pathological damage of pulmonary tissue in the sivelestat sodium treated group was between the control group and the severe acute pancreatitis group.Conclusion The application of sivelestat sodium in earlier period can play definite role for the treatment of severe acute pancreatitis.Its possible mechanisms maybe that sivelestat sodium play a role of relieving severe acute lung injury by inhibiting the activity of NF-κB.