Protective Effects of Sivelestat on Acute Liver Injury after Severe Acute Pancreatitis in Rats

Objective:To study the role of Sivelestat in the hepatic injury in rats with severe acute pancreatitis(SAP).Methods:Fifty-four SD rats were randomly divided into three groups,control group(group A),severe acute pancreatitis group(group B) and treatment with Sivelestat group(group C).Each group was subdivided into 3 h,6 h and 12 h subgroups(n=6 in each subgroup).Rats of group A were only operated with a celiotomy.SAP was induced by retrograde injection of 3.5% sodium taurocholate into biliopancreatic duct in group B.Sivelestat was instilled continuously into right jugular vein with infusion pump in group C.The levels of serum interleukin-6(IL-6),neutrophil elastase(NE) and amylase(AMY) were measured.And the levels of superoxide dismutase(SOD) and malondialdehyde(MDA) in hepatic tissue were also measured.The pathological changes of hepatic and pancreatic tissues were observed by HE staining.Results:(1)The levels of IL-6,NE,AMY and MDA increased significantly(P 0.05),and SOD significantly decreased(P 0.05)in group B than those of group A.There were pancreatic edema,inflammatory cell infiltration,necrosis,liver sinusoidal congestion,cloudy swelling and necrosis of cells under microscopy.The injury aggravated with time extended.(2)The serum levels of IL-6,NE and AMY,and the level of MDA in liver tissue,decreased significantly in group C than those of group B(P 0.05),but the level of SOD increased significantly.There was a reduced degree of liver damage in group C.Conclusion:The level of NE was inhibited by treatment with Sivelestat,which could markedly attenuate the hepatic injury in SAP by effectively enhancing the oxygen free radical scavenging capacity and inhibiting inflammatory reaction.