2013•Tianjin yiyaoRequires access
Effects of Celecoxib on the Cell Migration, MMP-9 and VEGF Expression in Human Gastric Cancer SGC-7901
Wei Yongxi
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Abstract
ObjectiveTo observe selective cyclooxygenase-2(COX-2) inhibitor celecoxib on the cell migration,MMP-9 and VEGF expression of human gastric cancer SGC-7901.MethodsIn vitro culture of SGC-7901 cells,the cells were divided into blank control group and celecoxib treatment group(different concentrations of celecoxib 25,50 and 100 μmol/L).SGC-7901 cells were treated with celecoxib for 24 h.The effect of celecoxib on cell migration of SGC-7901 was detected by the damage repair experiment.RT-PCR assay was used to detect the effect of celecoxib on MMP-9 mRNA and VEGF mRNA expressions in SGC-7901 cells.ResultsThe migration distance of SGC-7901 cells was decreased with the increased concentration of celecoxib.There were significant differences between medium-dose and high-dose celecoxib groups and the control group.RT-PCR assay showed that MMP-9 mRNA and VEGF mRNA expressions in gastric cancer SGC-7901 were significantly decreased with the increased concentration of celecoxib,and there was a significant difference compared with those of control group(P0.05).ConclusionCelecoxib can inhibit migration of human gastric cancer SGC7901 cells,which may be related to the inhibition of COX-2 downstream of MMP-9 and VEGF expressions.