Effect of inhibitor of cyclooxygenase-2 on the expression of VEGF in A549 cell lines of non-sman cell lung cancers

Objective To study the effect of inhibitor of cyclooxygenase-2 on the expression of vascular endothelial growth factor(VEGF)in A549 cell lines of non-sman cell lung cancers. Methods The proliferation of A549 lung carcinoma cells was measured by MTT assay After A549 cells were treated with different dose celecoxib; After A549 cells were treated with celecoxib 50μmol/L for 48 h, the level of VEGF m RNA was detected RT-PCR, and the expressions of VEGF protein was detected by western blot method. Results Celecoxib could inhibit the proliferation of A549 cell by a time-and dose-dependent manner. Compared with control group, the level of VEGF m RNA was decreased significantly detected by RT-PCR after A549 cells were trealed with celecoxib 50μmol·L-1for 48 h, P0.01 and the expression of VEGF protein was decreased significantly detected by western blot method, P0.01.Conclusion celecoxib might inhibite the proliferation of A549 lung carcinoma cells by regulating the expressions of VEGF.