Modulating effect of celecoxib to suppress proliferation and the molecular mechanism in caco-2 of colon carcinoma cell line

Objective To study celecoxib in vitro human colon cancer cell growth and proliferation of Caco-2 and its anti-tumor molecular mechanisms.Methods Cultured human colon cancer Caco-2 were divided to two group(one group is the control group that has no interference,the other group is celecoxib interference group).MTT method was used to analyse celecoxib at the same concentration,at different times for the colon cancer cell proliferation and calculate IC50 value.RT-PCR method was used to evaluate the expression of COX-2,MMP-9 mRNA.Results MTT assay showed the concentration of the same intervention celecoxib to inhibit the proliferation of human colon cancer cells,its 24 h,48 h,72h IC50 of respectively: 99.519 ± 10.355μmol / L,71.546 ± 6.446 μmol / L,59.622 ± 15.999 μmol / L.The RT-PCR results show that human colon cancer cells Caco-2 normal control group and celecoxib in the intervention group,respectively,the COX-2mRNA gray value for: 0.873 ± 0.026,0.115 ± 0.008,P 0.05.MMP-9 mRNA gray values were: 0.808 ± 0.021,0.101 ± 0.002(t = 19.037,P = 0.000).MMP-9 mRNA were 0.798 ± 0.031,0.190 ± 0.002(t = 18.987,P = 0.002).Conclusion Celecoxib can inhibit gastric cancer cell proliferation.Celecoxib antitumor mechanisms may by inhibiting the expression of COX-2 and MMP-9 mRNA in implementation.