Expression of HBV-DNA in the Umbilical Cord Tissues of The Dead Fetus Delivered From Puerperant With Hepatitis Virus B Infection

Objective To observe the expression of HBV-DNA and whether or not copy of HBV in the umbilical cord tissues of the died fetus delivered from the puerperant with hepatitis virus B infection.Methods 40 cases of died fetus were collected,and made the autopsy,we detected HBV-DNA in umbilical cord tissues with in situ molecular hybridization technique. HBV was detected in the mother veins before delivery.Results For the umbilical cord tissues, there were 16/40(40%) cases detected out HBV-DNA.When the prenatal sera HBV were high,low and without replication status,the detectable rates of HBV-DNA in the umbilical cord tissues were 1/4(25%)cases,7/20(35%)cases and 8/16(50%) cases respectively;For the detectable rates of HBV-DNA in the umbilical cord tissues,the difference was not statistically significant among the high,low and without replication status (P0 05). HBV-DNA were mainly localized on the surface of the umbilical cord vessel and in the plasma of the cord vessel's endothelial cells. HBV-DNA in the cord vessel's endothelial cells nuclei was not seen.Conclusion There were HBV replication in the umbilical cord tissues of the died fetus. But expression of HBV-DNA in the umbilical cord tissues of the died fetus was not related with the HBV replication status in the pregnant woman veins.