Comparison of mesenchymal stem cells culture from premature fetus umbilical cord blood and umbilical cord

Objective To compare the achievement ratio of mesenchymal stem cells(MSCs) derived from premature fetus umbilical cord blood and umbilical cord,inorder to explore the best way of obtaining MSCs.Methods Umbilical cord blood were collected from premature fetus(less than 37 months) under aseptic condition,mononuclear cells were isolated by density gradient centrifugation,and MSCs was obtained by attachment culture method;umbilical cord was cut into 1 mm3 tissue block,MSCs were separated from which with tissue attachment culture method;the biological characteristics of MSCs from umbilical cord blood and umbilical cord were observed,and the cell surface markers were detected by immunofluorescence method.Result In MesencultTM medium,the achievement ratio of umbilical cord blood from preterm fetus was 100%,and which of the umbilical cord was 67%(P0.05).MSCs from umbilical cord blood and umbilical cord in preterm fetus both expressed CD29,CD44 and CD90 abundantly,but not expressed the hematopoietic antigen CD34.Conclusion It is easy to obtain MSCs from umbilical cord blood in premature fetus,which provides ideal source for clinical cell transplantation.