Relationship between single nucleotide polymorphisms of -460T/C in VEGF gene and the susceptibilitiy of lung cancer in Hebei province

OBJECTIVE: To investigate the relationship between single nucleotide polymorphisms of -460T/C in the promoter region of VEGF gene and the susceptibilitiy of lung cancer in Hebei province.METHODS: This hospital-based case-control study included 200 lung cancer patients and 204 healthy controls.Genomic DNA was extracted by using proteinase K digestion followed by a salting out procedure.Polymorphisms of VEGF gene were analyzed by PCR-restriction fragment length polymorphism analysis(PCR-RFLP) and primer-introduced restriction analysis PCR(PIRA-PCR).RESULTS: The frequency of the VEGF-460 C/T SNP C allele in lung cancer patients(27.5%) was significantly higher than that in controls(20.1%,χ2=6.109,P=0.013).The frequencies of three genotypes(T/T and C/T+C/C) in controls were 63.2% and 36.7% respectively,and in lung cancer patients were 52.2% and 47.5% respectively.There was significant difference between the two groups(χ2=4.445,P=0.029).Compared with individuals with the T/T genotype,individuals with the T/C+C/C genotype had significantly higher risk to develop lung cancer.Compared with individuals with the T/T genotype,the T/C+C/C genotype increased the risk in developing lung cancer in non-smokers.When stratified by tumor histology,compared with individuals with the T/T genotype,individuals with the T/C+C/C genotype carried an increased risk of lung cancer in squamous cell carcinomas and small cell lung carcinomas.CONCLUSION: VEGF -460T/C SNP is associated with the risk of lung cancer.