Effects of GAPSGF on Inhibiting Hepatoma Cell Line SMMC7721 Proliferation and CDK2 Expression

Objective: To study the effects of Ganoderma appanatum polysaccharides GF(GAPSGF)on proliferation and CDK2 expression in hepatoma cell line SMMC7721.Methods: MTT assay was used to examine the growth and proliferation of SMMC7721 cells after treatment with different concentrations of GAPSGF.Real-time PCR and Western blot were performed to measure the expression of CDK2 at mRNA and protein level.Results: GAPSGF could effectively inhibit proliferation of hepatoma cell line SMMC7721,and GAPSGF at concentration of 2.5μg·mL-1 and 10μg·mL-1 had a significant effect on inhibiting proliferation.The expression of mRNA and protein of CDK2 effectively reduced.Conclusion: GAPSGF restrains proliferation of hepatoma cell line SMMC7721 by reducing CDK2 expression.